|
Post by lloyd on Feb 19, 2009 23:11:08 GMT -5
|
|
|
Post by hackerberry on Feb 19, 2009 23:35:14 GMT -5
PM'd lloyd.
hb
|
|
|
Post by lloyd on Mar 15, 2009 22:20:27 GMT -5
I am the proud owner of ~3.3mg of 24-epibrassinolide along with dvg and hackerberrry. It costs $3000 per gram! You use it in a concentration of 5 ppb (parts per billion). So 5 mcg will make 1 liter at that concentration. I finally got my homemade scale as accurate as I think I can (for ~$20 it's pretty good even though I've been working on it forever), I can weigh to about 10 mcg. I just wieghed a typical table sugar crystal at around 30mcg. So now I can start making some stock solutions with this stuff.
|
|
|
Post by lloyd on Mar 16, 2009 12:04:17 GMT -5
This morning I took the tiniest, possible bit that was still actually visible of 24-epibrassinolide from the 3.3 mg in the bottle. I weighed it out to something around 420 mcg. Then I dissolved it in 95% ethanol and made it up to 50cc of distilled water. So it's ~8.4 mcg/cc.
I added 0.05 cc of the stock solution to 40cc 250ppm GA3 and I'm now using it on all my seeds that have been stratifying all winter. It should be around 10 ppb of the 24-epi.
Tomorrow, I'm going to try and make some TC medium with ~.005ppm of the 24-epibrassinolide.
|
|
|
Post by hackerberry on Mar 16, 2009 12:55:36 GMT -5
Keep us posted Lloyd. I thought the epi is soluble in water?
hb
|
|
|
Post by lloyd on Mar 16, 2009 13:29:46 GMT -5
Phytotechlab says it's soluble in water but some references suggest dissolving it in methanol or alchohol. I decided to try the ethanol and it worked.
|
|
|
Post by hackerberry on Mar 16, 2009 13:32:54 GMT -5
ok, I will try both.
hb
|
|
|
Post by lloyd on May 28, 2009 12:08:25 GMT -5
I noticed some activity in my TC. Somethings seem to be sprouting but not a lot. I have some weird green stuff in two containers which is not taking over the bottle, yet. Some of my VFT's look wan and others are going crazy. An odd, callousy thing in one flask-too much hormones, I guess.
My P. Pumila is really doing well in there, one is even flowering and it's only a few months old. I'm really busy with the new house and my outside CP's but I'll try and post some pictures (an old broken promise).
Got eight more flasks ready for seeding-7 after I spilled one.
|
|
|
Post by dvg on May 28, 2009 13:02:19 GMT -5
Sounds promising Lloyd. Let us know how they turn out for you.
|
|
|
Post by tom on May 28, 2009 13:12:40 GMT -5
oohhh keep us updated
|
|
|
Post by hackerberry on May 28, 2009 16:19:31 GMT -5
Pictures Lloyd! You're getting us too excited! Anyways, here are some of the ex-vitro D. Burmanni around 3 months old. Still maturing VFTs from DVG. Too much hormones I guess? hb
|
|
|
Post by ellsie on May 28, 2009 16:29:40 GMT -5
Wow Hackerberry, Those ex-vitro D. Burmanni look sooo cool!! I've never seen that plant with white tentacles before.....or is that just because these plants are TC grown??
|
|
|
Post by hackerberry on May 28, 2009 16:35:29 GMT -5
Hi ellsie, they will turn a little reddish when exposed to the sun. I like them coz they are one of the sundew species that reacts/traps fast. Thanks!
hb
|
|
|
Post by lloyd on May 28, 2009 22:15:25 GMT -5
I don't want to excite anybody! There's just a few specks besides the VFT's and the P. Pumila. My failures at TC are legion. Those weird VFT's look like the ones I tried with hormones, all twisted and weird and (mostly) black and dead.
Still I do have a few interesting things which I will try and post.
|
|
|
Post by lloyd on Oct 27, 2009 13:36:12 GMT -5
|
|