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Post by hackerberry on Jan 14, 2009 16:12:35 GMT -5
Did you guys user PPM when you replate?
hb
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Post by dvg on Jan 14, 2009 16:41:26 GMT -5
I have tried 3 sources of ceph seed up to now. Only the seed from Insektenfang-the group order that Sib arranged, germinated - 4/12. One seedling aborted after germinating. I think most ceph (and nep) seed is old and non-viable. One is now out of TC. Two are still in TC, one is like a miniature ceph jungle & one is doing Ok but not as well. I'm contemplating on taking a few leaf pulls from the ones in TC and replating them in a ceph recommended hormone enriched medium. However I'm scared I will kill the original plant and the explants will die anyway. What does the group think I should do? Lloyd, that's a good question and also a tough call. The fact that these seeds are so hard to germinate and often non-viable when coming from afar, makes your three plants very special. Also because these are seed raised, they are all genetically distinct. Each one of these is unique in ways clones never are. The one that is lagging now, might really take off in real soil and fresher air, once deflasked. Now, if you really wanted to try replating some new cuttings, I would suggest the one to try is the one that is still lagging, because if that was lost, you'd still have the best two out of three left. Two outta three ain't bad.
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Post by hackerberry on Jan 14, 2009 16:45:21 GMT -5
I agree with dvg on this one. Might be time to pot the lagging one. Still awesome to see that the seeds germinated.
hb
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Post by lloyd on Jan 14, 2009 18:22:04 GMT -5
The runty one is the one in soil now. It's too traumatized and small to take cuttings from. I may take a cutting from the medium one in TC and wait to see if there's contamination.
I did use 0.1% PPM in the second bottle but I didn't do a PPM soak before re-plating as I thought it was sterile.
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Post by lloyd on Jan 14, 2009 18:45:16 GMT -5
Ceph initiation medium:
Full MS Vitamins
1/2 strength MS
Per liter:
1 mg BAP
0.1 mg IBA
30 gm. sucrose
0.1% PPM
100 mg. Casein
7 gm agar
pH to 5.5-5.8
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Post by dvg on Jan 14, 2009 18:58:32 GMT -5
Just curious, Lloyd, but what is the concern with an in-vitro ceph donating cuttings? If the sterile protocol is successfully followed, what is the danger to the mother plant. Do tc'ed plants tend to bleed out and dessicate if cut in their protective womb?
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Post by lloyd on Jan 14, 2009 19:30:06 GMT -5
dvg:
I once killed a D. derbyensis by taking some pullings when I took it out of TC. The pullings died and the donor plant went into shock and died. So I'm always leery of playing around with them. But I'm willing to take a chance on one of them.
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Post by dvg on Jan 14, 2009 20:15:06 GMT -5
If you left the medium plant in-vitro for a while yet, would it continue to grow larger? Was thinking a larger, more robust plant would not only offer larger sized cutting material, but also provide the donor plant with a sturdier constitution, hopefully better equipped to survive and thrive, after dealing with the cuttings and transplant stresses.
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Post by lloyd on Jan 14, 2009 20:46:23 GMT -5
I'm itching to try a cutting. Maybe just one little one to test for contamination.
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Post by sdeering on Jan 14, 2009 20:50:46 GMT -5
Thanks Lloyd
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Post by dvg on Jan 14, 2009 21:03:23 GMT -5
I'm itching to try a cutting. Maybe just one little one to test for contamination. You're the boss.
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Post by lloyd on Jan 15, 2009 17:14:03 GMT -5
I gently took 3 miniscule pitchers off the smaller plant and plated them. Mould usually becomes evident by the first 10 days so I'll know soon.
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Post by hackerberry on Jan 15, 2009 17:40:23 GMT -5
Update us with the results lloyd, I hope everything will be ok. Did you dip them in PPM?
hb
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Post by lloyd on Jan 15, 2009 20:53:03 GMT -5
I was thinking of the PPM dip but I was afraid it might be a bit toxic. If these grow mould my next ones will be soaked in 4%PPM for a few hours.
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Post by hackerberry on Jan 15, 2009 23:38:02 GMT -5
One of the tc bottle was infected with mould 1 month after the seed germinated. Mould was all over the place. I mixed a 4% PPM and pour some in the tc bottle. The mould was controlled, now I need to replate.
hb
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