My latest TC bottles don't seem to be doing too well, the seedlings look like they're fading. I noticed that the bottle of MS Salts I am using seems to have changed. The colour of the powder has gone from a creamy white to a slightly yellow off white. Also there is a distinct Chlorine smell to the container as if some of the chemicals have deteriorated. I didn't think it would go off if it was tightly sealed in the fridge. Should I throw this bottle away?
At the lab where i worked, our bottle was over 1 year and half old when we finished it. The powder was yellowish, but i didn't noticed any chlorine smell. It was kept closed in the fridge. I would try to make another batch of media and replate them, perhaps there was a slight error/difference in manipulation for that batch?
------------------------------------------------- "C'est une triste chose de songer que la nature parle et que le genre humain n'écoute pas." -Victor Hugo
Only a few jars left for the summer. One VFT seed culture that's almost filling the bottle. One bottle with two bits of B52 flower stem that are at least still green. And my pride and joy, 3 "large-type" cephs from seeds which look sort of half-plant, half callous. When they get bigger, I'll try to be brave and propagate them. All the rest, either died, or out in my microbogs, under lights or in the terrarium. Once I move (winter time), I'm going to get into TC again.
My "mg" scale came today in good time. The mg part is sort of a misnomer. It can measure an increment of 1 mg accurately. It measures a 10 g. reference as 10.003 gm which is pretty good for $25 with postage. However it is not able to accurately measure my 10 mg reference and reads it as 7mg. It cannot even react to my 1 mg. reference. I suspect that it cannot measure accurately below ~20 mg or so. Still for the money it's pretty good.
3 of my "Giant" ceph seeds grew in TC. They have been in the same bottles for more than a year now. One was yellowing. I decided to replate it with some hormones in a new bottle of media. Using as sterile technique as I could I transfered it to the new bottle. Within a week there was mould contamination clearly emanating in a spherical distribution around the plant. I took it out, carefully washed all the agar off and planted it. In a day there was mould growing. I treated it with No-Damp. I hope it will be Ok.
Of interest, the agar was completely clear of mould everywhere except the area directly next to the plant. It seems that the mould was from the plant, as far as I can see. I wonder if the mould could have been systemic in the ceph from the seed. Then the minor trauma could have released it into the new agar.
Lloyd, that's impressive work with the 'Giant' ceph seed. I have tried ceph seed before from RES and was unable to have anything germinate for me. Of course, with your seed being in tc, that gives your seeds a good head start and protection against various fungi. Good work!
I have tried 3 sources of ceph seed up to now. Only the seed from Insektenfang-the group order that Sib arranged, germinated - 4/12. One seedling aborted after germinating. I think most ceph (and nep) seed is old and non-viable.
One is now out of TC. Two are still in TC, one is like a miniature ceph jungle & one is doing Ok but not as well. I'm contemplating on taking a few leaf pulls from the ones in TC and replating them in a ceph recommended hormone enriched medium. However I'm scared I will kill the original plant and the explants will die anyway.
I have fond the same results with anything that has been in TC for a long time. Some VFT look good but when replated they do the same thing mold from the plant. I suspect it is the very low PH of the media that prevents it from growing out from the plant. I have tested some of the old media and it was 2.5. What is your protocol for Cepholotus? I might have to take some pullings of mine some day just for kicks. Stephen
dvg: Sarra's safe inside
Mar 31, 2019 20:40:58 GMT -5
bonfield: I've decided to finally reveal my best-kept secret for growing healthy Neps: Just spit on them every few days, the enzymes in saliva help them to better absorb the fertilizer I spray them with!
Apr 1, 2019 14:24:31 GMT -5
dvg: Salivating up your drools?
Apr 1, 2019 16:30:07 GMT -5
dvg: Duped again on April Fool's!
Apr 1, 2019 16:30:45 GMT -5