|
Post by exoticplantseller on Sept 27, 2016 5:36:32 GMT -5
ok the comments are harsh but realty , my advice is to look at some home culture products put out by Carol Stiff or similar that has a pack with all you need vessels media some tools ect ect , and go that way rather than any home style get any thing will do , that would be a very good start in right direction , may be get in contact with Frank www.youtube.com/channel/UCrkvUPW9QFLn2LtNhQvl01g , he good to know in this way too , know Frank for long time good person or contact Carol direct , think you will get where you want a lot quicker I was thinking about the kits, but never heard if anyone tried them or what the reviews where, now that I know they are good I might get it for Christmas or my birthday lol. I'm getting a little older now so it's time to stop asking for kid stuff for Christmas and ask for things I need. So maybe I will ask for some of these things. Lol
|
|
|
Post by sokkos on Sept 27, 2016 7:45:40 GMT -5
Contamination is the first opportunity to troubleshoot. I would say that you could first prep a couple bottles of media and then after you do your current sterilization procedure just cap them tight and let them sit there in what would be your grow space and see if after a few weeks anything unwanted appears. If nothing does than it means that your contamination is from the addition of non adequately sterilized seeds or whatever. If something does start growing in just plain media then your problem is at this very early step. If your prepped and sterilized media stays clean after a few weeks then that may not be the problem. Learning a stepwise troubleshoot is a big part of any experimental procedure and is worth the learning that comes with that regardless in my opinion. If you have patience and the process is cheap then there really isn't a lot to lose. If you get to a point where it just doesn't work despite your best efforts then it's time to stop and wait until you can get the right tools and training. The person that is helping me with this sent me a message and said to wait a week before planting to see if I get contamination, but I had already planted the seeds before I saw the message. Maybe I will make some media tonight and sterilize it. Would I have to let it cool and turn to a gel before I STERILIZE? Or can it be hot still and then I sterilize IT? Cause the media just melts a again anyways. I think waiting is very good (and logical) advice. Bacteria takes a few days to show up while fungus can take weeks. Leave the medium for two weeks before you do anything with it. If you cannot get sterile medium, it doesn't make sense to waste plant material. When I test containers, I usually leave them for about two months after sealing them. This is to check for contamination and airflow. Typically you measure out your liquid, adjust pH etc. then add the right amount of agar into a bottle; add the liquid medium to the bottle and swirl to mix; and then sterilize. I assume you won't be using a pressure cooker, so I guess the best way would be to bring the mixture to a rolling boil then pout it hot into the jars and cover them immediate without snapping on the lid. The containers should be not melt if they are polypropylene (check for a triangle with the number 5 inside and "pp" underneath the triangle). Often polypylene containers have lids made with other types of plastic that WILL melt so check the material. Please be VERY VERY careful because boiling agar splatters and can foam up.
|
|
|
Post by exoticplantseller on Sept 27, 2016 15:38:01 GMT -5
The person that is helping me with this sent me a message and said to wait a week before planting to see if I get contamination, but I had already planted the seeds before I saw the message. Maybe I will make some media tonight and sterilize it. Would I have to let it cool and turn to a gel before I STERILIZE? Or can it be hot still and then I sterilize IT? Cause the media just melts a again anyways. I think waiting is very good (and logical) advice. Bacteria takes a few days to show up while fungus can take weeks. Leave the medium for two weeks before you do anything with it. If you cannot get sterile medium, it doesn't make sense to waste plant material. When I test containers, I usually leave them for about two months after sealing them. This is to check for contamination and airflow. Typically you measure out your liquid, adjust pH etc. then add the right amount of agar into a bottle; add the liquid medium to the bottle and swirl to mix; and then sterilize. I assume you won't be using a pressure cooker, so I guess the best way would be to bring the mixture to a rolling boil then pout it hot into the jars and cover them immediate without snapping on the lid. The containers should be not melt if they are polypropylene (check for a triangle with the number 5 inside and "pp" underneath the triangle). Often polypylene containers have lids made with other types of plastic that WILL melt so check the material. Please be VERY VERY careful because boiling agar splatters and can foam up. Yes, I have noticed the splattering when boiling it. I was thinking it wasn't normal but I guess it is. I sterilized in the microwave, probably not hot enough, but it overflowed out of the jars a little, so I learned to do intervals of 10 seconds until it starts to foam. Thanks for all the help I will keep these tips in the vault.
|
|
|
Post by snapperhead51 on Oct 6, 2016 22:15:19 GMT -5
some good advice indeed, all though now days i don't wait to see if u got jars contaminated , it was when i started very prudent to do so , now i let me jars cool from the autoclave strait in to my flow cabinet running of cause to stop any jars that leak or draw air in will have only 100% pure air sucked in and elimination any contamination well 99.9 % of it . media splatter is not good on the inside of your jar with will cause the contamination you want to avoid , especially around the lid area ,where most air born contams start , boiling your jars with media in, is not a good idea as splatter will become a problem, and over cooking of the media can cause the media to become caramelized or spoil , over cooking will also take out many of the PGR's or other things you may have put in to enhance your growth or rooting of your cultures , as the strength of any additive will lessen with sterilization of the media , is where you can add PGR's and additives with micro filter sterilization after the jars have been autoclaved ,but all producer's must be to 100% sterilization process in this process all can go wrong very quickly. Some jars need to be vented to work well where others don't , make sure you know what your jars are and how they work ,very important , many go to smaller jars when in-fact they should be using bigger jars , go on the larger side ,you efforts will be rewarded . while the microwave can sterilize your jars with media in,the media loss is quite extensive and temps and times are critical,over cooking is very easy to do , a large pressure cooker is probably a better choice ,your jars need to be sterilized at 14 to 15 psi no more and 119 to 120 c for min of 20 min with a 12 min pressure release ,(very slowly cool down) to avoid jars exploding on you inside the sterilizer. of cause the proper autoclave is best but are not cheap well not here any how ,but is the best way to go is your any where serious .if your using glass jars with tin twist lids, using double filter paper under the the lids is a must ,the use of coffee filters is a cheap way and works well , just place lid on the filter draw round it cut it out and place in inside of lid , and you have a filtered lid hope that helps
|
|
|
Post by exoticplantseller on Oct 7, 2016 6:41:32 GMT -5
Thanks you. I am trying to find a pressure cooker. Will the jars need to be somewhat open when in the pressure cooker? And will they still SPLATTER? Thanks
|
|
|
Post by lloyd on Oct 7, 2016 9:46:11 GMT -5
I deleted the quote because it was a needless repetition. Try to have quotes be short and meaningful. Long quotes and quotes with graphics, clutter up a thread with no added content.
Second of all, don't get a pressure cooker. Don't do TC by yourself. Get a responsible, knowledgeable adult to help you and guide you and protect you.
Please don't ask any more questions about TC on this forum until you have followed the above advice. We don't want to see young people harming themselves when they don't safely follow the informative and well meant advice being offered.
|
|
|
Post by exoticplantseller on Oct 7, 2016 15:04:16 GMT -5
Hi lloyd, I have a parent helping me with the boiling and sterilizing. Thanks for watching out for me. This thread is very clustered. Should it be Deleted? Thanks for the advice
|
|