|
Post by exoticplantseller on Sept 22, 2016 14:41:31 GMT -5
Ok I will try to find it
|
|
|
Post by snapperhead51 on Sept 26, 2016 8:05:52 GMT -5
Doing T/c is not easy if you wish to do it right , its time consuming and expensive , using home style media and agar's ect and cheap culture vessels will only lead to disappointment in the end , seen it many times over people all enthused and keen to find 12 months down the road all has gone very wrong and most cultures contaminated or not growing , learning what media and PGR ratios and sugar ratios and many other requirements takes some times years to archive to grow different cp's its not 1 media for all. you relay need to do it right to do well or not at all ,do i do T/c yes a lot of it in my own lab ,which to do right is the best and most rewarding way , you all so need a lot of patients and $$ to make it do well trust me if i/ you want to grow 3 VFT's a few sarras and a heli here and there u mite do ok , but to produce good amounts a cultures you will need suitable conditions , lighting , climate control and lab requirements , tools and autoclave and proper flow cabinet and the correct culture vessels with suitable lids ,sorry no other way as far as I'm concerned with T/c John
|
|
|
Post by RuBisCO on Sept 26, 2016 12:11:32 GMT -5
I think it is a good thing to try things of this sort. There are lots of benefits to it even if there is complete failure. As long as something like this is done safe then it provides much opportunity for one to problem solve, trouble shoot, research etc. Certainly no harm in doing it if one is aware that it is a very tricky and technical process that really should be done in a lab environment. If it is fun doing it then have at it. Better than video games! Cheers
|
|
|
Post by exoticplantseller on Sept 26, 2016 15:20:19 GMT -5
Doing T/c is not easy if you wish to do it right , its time consuming and expensive , using home style media and agar's ect and cheap culture vessels will only lead to disappointment in the end , seen it many times over people all enthused and keen to find 12 months down the road all has gone very wrong and most cultures contaminated or not growing , learning what media and PGR ratios and sugar ratios and many other requirements takes some times years to archive to grow different cp's its not 1 media for all. you relay need to do it right to do well or not at all ,do i do T/c yes a lot of it in my own lab ,which to do right is the best and most rewarding way , you all so need a lot of patients and $$ to make it do well trust me if i/ you want to grow 3 VFT's a few sarras and a heli here and there u mite do ok , but to produce good amounts a cultures you will need suitable conditions , lighting , climate control and lab requirements , tools and autoclave and proper flow cabinet and the correct culture vessels with suitable lids ,sorry no other way as far as I'm concerned with T/c John I am just doing this as an experiment and fun. Not looking to mass produce anything, just something to do and learn. I did try and i got a white stringy mold contamination and in the other jar an algae contamination. A person that thought me said I didn't sterilize enough so I am going to try again next week. If I have success I will let you all know. I have a lot of patience and thought this would be a cool thing to try and fail or maybe success. I like experimenting with this kind of stuff. Maybe in a while I'll have success haha
|
|
|
Post by exoticplantseller on Sept 26, 2016 15:22:20 GMT -5
I think it is a good thing to try things of this sort. There are lots of benefits to it even if there is complete failure. As long as something like this is done safe then it provides much opportunity for one to problem solve, trouble shoot, research etc. Certainly no harm in doing it if one is aware that it is a very tricky and technical process that really should be done in a lab environment. If it is fun doing it then have at it. Better than video games! Cheers Haha your right. I totally agree with you. I find experimenting with this stuff very interesting. I am just doing it for fun, so I don't care if I fail a few times. Maybe in the future I will learn and be good at this. You are right about the video games. I don't play much myself hah
|
|
|
Post by lloyd on Sept 26, 2016 16:52:46 GMT -5
You will gain a good appreciation of mould and bacteria.
|
|
|
Post by exoticplantseller on Sept 26, 2016 17:17:55 GMT -5
You will gain a good appreciation of mould and bacteria. Haha lol I already have 😂
|
|
|
Post by snapperhead51 on Sept 26, 2016 18:26:21 GMT -5
Yes well you may find my comments harsh or not appealing , I am speaking from experience ,I to wanted to dabble in T/c thinking i just do a few jars here and there with home brews ect ect , sorry didn't work , all i got was a lot of time and effort spent doing stuff that didn't work , in the end i had to conform to the proper protocols ,methods and equipment , while you can make the flow box , and use other things for autoclave, the tools jars and media, sterilization,procedures must be perfect every time 100% in T/c there is no short cuts , or all you get a jars full of contamination and dying cultures ! 1 very important thing to know , you cant get sterilization credits, its either sterile or its not !! , most have to lean the hard way on this point trust me! the other thing is there is not a lot of real information on CP T/c ing out there , and most like me don't want to give many years to trail and error information away for nothing ,been a hard slog , when i started most you got was about 1/2 the info needed to get thing to grow , the rest had to be working out from costly , painful time consuming trails , to get a cultures to grow , you be looking at about a 2 year time frame to get any results from scratch . growth with T/c is faster is still slow no matter what u do John
|
|
|
Post by lloyd on Sept 26, 2016 18:57:15 GMT -5
Sterilizing and proper flow hood technique is critical.
|
|
|
Post by Dennis Z on Sept 26, 2016 21:30:27 GMT -5
No harm in trying it exoticplantseller, just be sure to stay positive regardless of the outcome.
I really hate to be discouraging anyone from trying new things however, this is a public forum and so, making tissue culture sound like a "fun and new" activity is a recipe for disaster.
|
|
|
Post by RuBisCO on Sept 26, 2016 22:05:44 GMT -5
Contamination is the first opportunity to troubleshoot. I would say that you could first prep a couple bottles of media and then after you do your current sterilization procedure just cap them tight and let them sit there in what would be your grow space and see if after a few weeks anything unwanted appears. If nothing does than it means that your contamination is from the addition of non adequately sterilized seeds or whatever. If something does start growing in just plain media then your problem is at this very early step. If your prepped and sterilized media stays clean after a few weeks then that may not be the problem. Learning a stepwise troubleshoot is a big part of any experimental procedure and is worth the learning that comes with that regardless in my opinion. If you have patience and the process is cheap then there really isn't a lot to lose. If you get to a point where it just doesn't work despite your best efforts then it's time to stop and wait until you can get the right tools and training.
|
|
|
Post by snapperhead51 on Sept 27, 2016 0:27:44 GMT -5
ok the comments are harsh but realty , my advice is to look at some home culture products put out by Carol Stiff or similar that has a pack with all you need vessels media some tools ect ect , and go that way rather than any home style get any thing will do , that would be a very good start in right direction , may be get in contact with Frank www.youtube.com/channel/UCrkvUPW9QFLn2LtNhQvl01g , he good to know in this way too , know Frank for long time good person or contact Carol direct , think you will get where you want a lot quicker
|
|
|
Post by exoticplantseller on Sept 27, 2016 5:27:47 GMT -5
Yes well you may find my comments harsh or not appealing , I am speaking from experience ,I to wanted to dabble in T/c thinking i just do a few jars here and there with home brews ect ect , sorry didn't work , all i got was a lot of time and effort spent doing stuff that didn't work , in the end i had to conform to the proper protocols ,methods and equipment , while you can make the flow box , and use other things for autoclave, the tools jars and media, sterilization,procedures must be perfect every time 100% in T/c there is no short cuts , or all you get a jars full of contamination and dying cultures ! 1 very important thing to know , you cant get sterilization credits, its either sterile or its not !! , most have to lean the hard way on this point trust me! the other thing is there is not a lot of real information on CP T/c ing out there , and most like me don't want to give many years to trail and error information away for nothing ,been a hard slog , when i started most you got was about 1/2 the info needed to get thing to grow , the rest had to be working out from costly , painful time consuming trails , to get a cultures to grow , you be looking at about a 2 year time frame to get any results from scratch . growth with T/c is faster is still slow no matter what u do John Lol these are good points. I knew about this when I was starting. I don't plan on doing it with cp's for a while maybe easy things. And then when I branch out the money for proper ms media and proper sterilization and a glove box and all that kind of stuff I will try with CPs. But I don't have enough seeds or cuttings to try with cps
|
|
|
Post by exoticplantseller on Sept 27, 2016 5:30:12 GMT -5
Sterilizing and proper flow hood technique is critical. That's what it seems like haha. And also I have been wondering, what is the picture of that comes with all your POSTS? And also maybe delete this chat this afternoon cause it is getting a bit crowded. Thanks
|
|
|
Post by exoticplantseller on Sept 27, 2016 5:33:37 GMT -5
Contamination is the first opportunity to troubleshoot. I would say that you could first prep a couple bottles of media and then after you do your current sterilization procedure just cap them tight and let them sit there in what would be your grow space and see if after a few weeks anything unwanted appears. If nothing does than it means that your contamination is from the addition of non adequately sterilized seeds or whatever. If something does start growing in just plain media then your problem is at this very early step. If your prepped and sterilized media stays clean after a few weeks then that may not be the problem. Learning a stepwise troubleshoot is a big part of any experimental procedure and is worth the learning that comes with that regardless in my opinion. If you have patience and the process is cheap then there really isn't a lot to lose. If you get to a point where it just doesn't work despite your best efforts then it's time to stop and wait until you can get the right tools and training. The person that is helping me with this sent me a message and said to wait a week before planting to see if I get contamination, but I had already planted the seeds before I saw the message. Maybe I will make some media tonight and sterilize it. Would I have to let it cool and turn to a gel before I STERILIZE? Or can it be hot still and then I sterilize IT? Cause the media just melts a again anyways.
|
|