I have been back into tissue culture for about 1 year now. Over that time, I have been slowly adding to the plants I have in culture. Most of the plants have been put into sterile culture from seeds but a few from flower stalks and leaf pulls. I purchased some seeds online but most of the material has been obtained from people through this board.
The cultures all appear to be stable right now and it looks like I will have more time in the new year to begin multiplying the plants. I will also get around to putting more plants in culture from my collection plus some of the extra seeds I have collected. I am also waiting on other seeds to germinate but it may be a lost cause if they were not fresh when I put them into culture.
I also lost most of the flytraps cultures from flowerstalks when my greenhouse went above 40 degrees in the summer. I am planning to monitor and control the greenhouse with an Arduino this growing season.
Nepenthes maxima N Lowii N veitchii N stenophylla
Heliamphora minor H nutans
Sarracenia "red tube" alata S flava x oreophila
Drosera binat D indica D peltata D auriculata D capensis "giant" D hilaris D binata "marston giant" D filiformis "California sunset" D. lowriei
Flytraps B52 DC XL Dentate Patches Red Dragon Typical Phalanx Flaming Lips Red Piranha Big Mouth
I tried TC with rare african violets...mostly went well until I had to transfer to growing out containers...guess I was not sanitary enough, turned brown and collapsed forever waiting to try TC again, with several plant species!
Post by snapperhead51 on Dec 17, 2015 18:03:37 GMT -5
Acclimatization of T/c plants is critical and often extremely hard to get right , each plant can need different methods to get acclimatized , soft plants are the worst indeed to get right , most orchids are very easy , most CP's are reasonably difficult , think the hardest is helis my self , and VFT's probably the easiest.
Post by amanitovirosa on Dec 17, 2015 19:52:00 GMT -5
...I got some TC Cephs off eBay a while back (know absolutely nothing about TC myself). Two batches, one completely died off and I got nothing but I was lucky with the other one and got a bunch of strikes. In total there were probably several hundred tiny TC Cephs, out of those I probably have about 40-50 that survived the transition to media and are growing. I'm really hoping for some mutations. Nice list, if you will be selling any I'd be interested in some. Cheers!
I have successfully deflasked VFT, Nepenthes and Heliamphora in the past. I have put all my Nepenthes inside under lights and only heat my greenhouse to keep it above freezing. When I get my greenhouse controls going in the spring, I will think about trying to grow some of the plants out. I have put some plants like the DC XL into multiplication medium and should have quite a few extras. These where seeds from a DC XL x DC XL so there is no telling whether these will be as large as a plant vegetatively propagated. I don't know about selling any of these plants but I am always up for trades. I do owe some people who sent me plant materials some of the offspring.
dvg: Spring is sprung, our grass ain't riz,
Mar 20, 2019 16:58:22 GMT -5
dvg: below the snow, asleep it is.
Mar 20, 2019 16:59:02 GMT -5
dvg: Still grassroots dream of their rise,
Mar 20, 2019 16:59:40 GMT -5
dvg: in sunny days, on blue skies
Mar 20, 2019 17:00:13 GMT -5
insectivore: Then Boom we Get hit with yet another Snow Storm in Quebec...
Mar 23, 2019 8:19:19 GMT -5
dvg: Parts of Quebec got hit with a lot of snow.
Mar 23, 2019 20:29:10 GMT -5
dvg: These late March snow storms are easier to digest than the mid-May wintry blasts that do so much damage to the trees
Mar 23, 2019 20:33:09 GMT -5
insectivore: Agreed! we got a good 10-12 inches but the warm days are taking care of it!!
Mar 27, 2019 18:04:28 GMT -5
dvg: That was a decent dump of snow!
Mar 28, 2019 8:49:24 GMT -5
dvg: With the days now longer than the nights and the noonday sun rising higher in the sky each day, it won't be long before winter is just an afterthought
Mar 28, 2019 8:53:16 GMT -5
lloyd: The plants wandered out of the garage and zoomed back in before a dump of snow.
Mar 31, 2019 19:21:44 GMT -5
dvg: landscapes draped in snow
Mar 31, 2019 20:39:55 GMT -5
dvg: Sarra's safe inside
Mar 31, 2019 20:40:58 GMT -5
bonfield: I've decided to finally reveal my best-kept secret for growing healthy Neps: Just spit on them every few days, the enzymes in saliva help them to better absorb the fertilizer I spray them with!
Apr 1, 2019 14:24:31 GMT -5
dvg: Salivating up your drools?
Apr 1, 2019 16:30:07 GMT -5
dvg: Duped again on April Fool's!
Apr 1, 2019 16:30:45 GMT -5