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Post by micropropagador on Nov 2, 2015 20:14:40 GMT -5
Hello fellows. I´ve been tryong to grow Drosera affinis and Drosera tomentosa in vitro. They germinate but die. I´ve been using MS, 1/2MS and 1/3 MS but with no goood results the seedlings start to get yrllow, than brown a and die. Do You guys have any clue? Thanks
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Post by lloyd on Nov 2, 2015 20:24:08 GMT -5
Do you use MS-Vitamines?
Do you use hormones or PPM? they can be toxic to some plants.
MS 1/2 (with sugar) should be good.
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Post by micropropagador on Nov 4, 2015 13:38:59 GMT -5
Hello Lloyd! It´s been a long time since the last time I entered this forum. I hope I´ll be more produtive now. I use MS 1/2 with vitamines, I don´t use any hormones. Do You have tomentosa and affinis in vitro Lloyd?
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Post by lloyd on Nov 4, 2015 18:28:37 GMT -5
Not yet, but I have lots of seeds for TC when I get time.
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Post by pinhead on Nov 5, 2015 23:06:21 GMT -5
I had the same problem with sundew seeds with 1/2 MS. They would germinate then die. Flytraps were not a problem.
I have switched to 1/3 MS with vitamins and PPM and have had better results.
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Post by micropropagador on Nov 6, 2015 9:44:07 GMT -5
Thank you for the replay pinhead. Next time I´ll try something like that.
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Post by snapperhead51 on Nov 6, 2015 16:46:17 GMT -5
yep try a bit less sugar as well , 20 to 25 gms /L not 30 gm/L ,no need to use PPM is sterilization is good !. often to much sugar will cause soem problems in some CP's
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Post by micropropagador on Nov 6, 2015 21:14:47 GMT -5
good information as well snapperhead51, I´ll try that too
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