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Post by pinhead on Nov 22, 2014 0:01:10 GMT -5
My school was one of the recipients of the generous donations from Regina of the Urban Bog when they shut down. We received chemicals, equipment and plants in vitro that we could never afford to purchase. I have a group of students involved in a club who are now interested in both CPs and tissue culture. We are busy reflasking plants and have put some new varieties in culture through seeds I have obtained. Glovebox About 1/3 of the cultures - we are running out of room Flaming Lips Flytrap reflasked Drosera regia Drosophyllum lusitanicum Byblis liniflora Besides the 3 shown above, we have also successfully germinated Darlingtonia californica, Drosera multifida, Drosera burmanni, Drosera aliceae I am a little rusty with my sterile technique, but we have had zero contamination with the seeds. I am not sure about the age of our seeds, but the seeds were sown only 3 weeks ago and treatment with Gibberellic acid seems to have worked for the Drosophyllum and Darlingtonia. Today we put into culture Drosera indica , Byblis lamelleta, DC XL and Big Mouth Flytrap seeds (supposedly hand pollenated, so hopefully they breed true) I also have some Cephalotus follicularis , Utricularia longifolia, Utricularia alpine, Byblis gigantea and Nepenthes truncata seeds in the mail. We will move onto explants when I can find a source. In the spring, if any one has any flytrap flower stalks from different cultivars we would love to try them. We would send you back some of your plants in culture if we were successful.
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Post by shoggoths on Nov 22, 2014 6:53:30 GMT -5
Nice project and really good works.
I didn't know we could do drosophyllum and byblis in TC. Let us know how the transplantation end with those please.
Are you only interested in VFT seeds ?
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Post by lloyd on Nov 22, 2014 16:03:05 GMT -5
I had some byblis go crazy in TC. Do you use PPM with your medium?
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Post by pinhead on Nov 22, 2014 19:56:37 GMT -5
We used 8% PPM to sterilize the seeds for 4 hours with an additional 1mL of PPM added to 1L of the medium. This has resulted in no contamination. When I originally tried tissue culture years ago, I started with African Violets leaves sterilzed in bleach and then peroxide. Sterilizing seeds was always hit and miss because I could never determine the correct amount of time to leave them in the bleach bath. It was either not enough time and they would still be contaminated or too long and it would kill the seed.
A few students are looking at a Science fair project testing sterilization of both seeds and tissue with PPM or NaDCC with various concentrations and times.
We would try any types of CP seeds or leaves/shoots or rhizomes. Plants will be given away to the students at the end of the school year with cultures maintained for the upcoming year.
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Post by ng on Nov 22, 2014 20:30:51 GMT -5
I've litterally got thousands of Drosera seeds left from this season if you want them bub... a few different varieties
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Post by sokkos on Dec 4, 2014 23:53:58 GMT -5
Great work! Your sterile technique must not be too rusty if you are not getting contamination! For sundew seeds I've been using 6 mins in bleach with very good success. The only sundew seeds that I haven't been able to sterilize are D. omissa and I've tried twice: once fungus grew from a single seed so I tossed the plate and the second time nothing germinated after 3 months.
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