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Post by dToad on Apr 24, 2012 15:16:51 GMT -5
In my first tissue culture attempt, I used 7.5 gm per liter. It took more than six weeks for calluses to form, and the plantlets were very slow to grow. There are several reasons this can happen, one of which is too strong a gel. This binds the nutrients tightly, making it difficult for the culture to absorb them.
Depending on your source, the amount of agar to use is specified either as gm/l or a volume (smidgen or pinch) per jar. Here are commonly used concentrations:
5.5 - 7.5 gm/l Phytotech A296 documentation, Frank Tromble videos smidgen / 25 CC - flytrapcare forum pinch / 3 tbsp (44cc)- Home Tissue Culture pinch / 50 cc - flytrapcare forum
There appears to be a huge difference in these measurements. I weighed one tsp of Phytotech's A296 agar, and found it to be 2.4 gms. Converting the above to gm/l we have:
5.5 - 7.5 gm/l Phytotech A296 documentation, Frank Tromble videos 3.0 gm/l - smidgen / 25 CC - flytrapcare forum 3.4 gm/l - pinch / 3 tbsp (44cc)- Home Tissue Culture 3.0 gm/l - pinch / 50 cc - flytrapcare forum
smidgen = 1/32 tsp pinch = 1/16 tsp
The volumetric measurements fall in the range of 1/2 the weighed measurements. My next TC attempt will use 1-1/4 tsp or 3gm per liter.
Follow up notes: 1. 3gm/l raises the pH by 0.9 (5.4 -> 6.3). Before adding agar, pH should be 4.6 - 4.8 to finish at 5.5 - 5.7
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Post by xeno on Jul 29, 2012 5:24:28 GMT -5
When I used agar I took 4.5g - 5.0g per Litre. But now I use Gelcarin and it can be mixed when the liquid is cold easy to adjust the PH at the end then.
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