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Post by tom on Feb 15, 2008 23:15:25 GMT -5
congratulation! keep us posted
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Post by lloyd on Mar 13, 2008 21:55:51 GMT -5
My latest TC bottles don't seem to be doing too well, the seedlings look like they're fading. I noticed that the bottle of MS Salts I am using seems to have changed. The colour of the powder has gone from a creamy white to a slightly yellow off white. Also there is a distinct Chlorine smell to the container as if some of the chemicals have deteriorated. I didn't think it would go off if it was tightly sealed in the fridge. Should I throw this bottle away?
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Post by tom on Mar 14, 2008 6:35:02 GMT -5
At the lab where i worked, our bottle was over 1 year and half old when we finished it. The powder was yellowish, but i didn't noticed any chlorine smell. It was kept closed in the fridge. I would try to make another batch of media and replate them, perhaps there was a slight error/difference in manipulation for that batch?
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Post by lloyd on Aug 5, 2008 21:41:06 GMT -5
Only a few jars left for the summer. One VFT seed culture that's almost filling the bottle. One bottle with two bits of B52 flower stem that are at least still green. And my pride and joy, 3 "large-type" cephs from seeds which look sort of half-plant, half callous. When they get bigger, I'll try to be brave and propagate them. All the rest, either died, or out in my microbogs, under lights or in the terrarium. Once I move (winter time), I'm going to get into TC again.
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Post by lloyd on Dec 16, 2008 16:40:16 GMT -5
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Post by hackerberry on Dec 16, 2008 16:42:06 GMT -5
That looks reasonable. Nice catch lloyd.
hb
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Post by lloyd on Dec 18, 2008 21:10:10 GMT -5
I updated my home-made scale with a zero detector so a LED goes on when the scale is level, much easier to use now.
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Post by lloyd on Jan 5, 2009 15:51:37 GMT -5
My "mg" scale came today in good time. The mg part is sort of a misnomer. It can measure an increment of 1 mg accurately. It measures a 10 g. reference as 10.003 gm which is pretty good for $25 with postage. However it is not able to accurately measure my 10 mg reference and reads it as 7mg. It cannot even react to my 1 mg. reference. I suspect that it cannot measure accurately below ~20 mg or so. Still for the money it's pretty good.
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Post by hackerberry on Jan 5, 2009 17:44:48 GMT -5
Same here lloyd, their labels lie! Good thing mine converts to grain which is smaller so I get a rough estimate with my hormones.
hb
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Post by lloyd on Jan 5, 2009 19:03:57 GMT -5
I wonder if I can take mine apart and recalibrate it. It may have something to do with the "Tare" function too. I wonder if I can hack it.
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Post by lloyd on Jan 14, 2009 9:29:20 GMT -5
3 of my "Giant" ceph seeds grew in TC. They have been in the same bottles for more than a year now. One was yellowing. I decided to replate it with some hormones in a new bottle of media. Using as sterile technique as I could I transfered it to the new bottle. Within a week there was mould contamination clearly emanating in a spherical distribution around the plant. I took it out, carefully washed all the agar off and planted it. In a day there was mould growing. I treated it with No-Damp. I hope it will be Ok.
Of interest, the agar was completely clear of mould everywhere except the area directly next to the plant. It seems that the mould was from the plant, as far as I can see. I wonder if the mould could have been systemic in the ceph from the seed. Then the minor trauma could have released it into the new agar.
Weird.
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Post by dvg on Jan 14, 2009 11:40:54 GMT -5
Lloyd, that's impressive work with the 'Giant' ceph seed. I have tried ceph seed before from RES and was unable to have anything germinate for me. Of course, with your seed being in tc, that gives your seeds a good head start and protection against various fungi. Good work!
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Post by lloyd on Jan 14, 2009 13:45:21 GMT -5
I have tried 3 sources of ceph seed up to now. Only the seed from Insektenfang-the group order that Sib arranged, germinated - 4/12. One seedling aborted after germinating. I think most ceph (and nep) seed is old and non-viable.
One is now out of TC. Two are still in TC, one is like a miniature ceph jungle & one is doing Ok but not as well. I'm contemplating on taking a few leaf pulls from the ones in TC and replating them in a ceph recommended hormone enriched medium. However I'm scared I will kill the original plant and the explants will die anyway.
What does the group think I should do?
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Post by hackerberry on Jan 14, 2009 15:32:27 GMT -5
Awesome lloyd, we would love to see pictures. I guess the mold was systemic and was dormant. The mold could have been released due to the change of ph.
hb
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Post by sdeering on Jan 14, 2009 15:53:53 GMT -5
I have fond the same results with anything that has been in TC for a long time. Some VFT look good but when replated they do the same thing mold from the plant. I suspect it is the very low PH of the media that prevents it from growing out from the plant. I have tested some of the old media and it was 2.5. What is your protocol for Cepholotus? I might have to take some pullings of mine some day just for kicks. Stephen
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