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Post by exoticplantseller on Aug 20, 2016 18:44:36 GMT -5
I recently had an idea of using gel beads as media for TC. I thought if there was a way to melt them and add vitamins you could pour it into the TC containers and add water to hydrate and plant seeds/cuttings in it. Has anyone tried THIS? I don't know enough to to try it, but is there anyone that would give it a go?
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Post by RuBisCO on Aug 20, 2016 22:53:12 GMT -5
I haven't done any plant tissue culture - but I have done lots of animal tissue culture in the past. It is unlikely the contents of the beads will contain the right 'stuff' for plants growth needs. Generally tissue culture media is a mix of minerals and amino acids and other things that serve as nutrients for he tissue being cultured; as growing animal or plant material have specific requirements to generate new tissue it is unlikely the beads will serve that purpose. I suspect that the beads just contain a material that is hydrophilic and sucks up water. There are specific growth media that are out there that contain all the bits and prices of molecular goodies in variable quantities that when mixed with a gelatin agent such as agar will form a happy solid gob that a plant will be tricked into thinking is good dirt to grow in. I would imagine growing a plant in melted beads would be like asking an infant to grow up big and strong on a diet of cardboard! Just my two cents... Possibly equally sustainable growth media if it were melted down....
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Post by exoticplantseller on Aug 21, 2016 6:31:48 GMT -5
I haven't done any plant tissue culture - but I have done lots of animal tissue culture in the past. It is unlikely the contents of the beads will contain the right 'stuff' for plants growth needs. Generally tissue culture media is a mix of minerals and amino acids and other things that serve as nutrients for he tissue being cultured; as growing animal or plant material have specific requirements to generate new tissue it is unlikely the beads will serve that purpose. I suspect that the beads just contain a material that is hydrophilic and sucks up water. There are specific growth media that are out there that contain all the bits and prices of molecular goodies in variable quantities that when mixed with a gelatin agent such as agar will form a happy solid gob that a plant will be tricked into thinking is good dirt to grow in. I would imagine growing a plant in melted beads would be like asking an infant to grow up big and strong on a diet of cardboard! Just my two cents... Possibly equally sustainable growth media if it were melted down.... What your saying does make sense, however, if you can find a way to add nutrients and things to the polymer beads and shape them to fit the TC container it could work.
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Post by micropropagador on Dec 4, 2016 7:12:35 GMT -5
I´ve tried this once. I used the beads with MS midia salts and water. What happened is that the beads hadn´t absorbed the water and I think it is because the midi8a was hipertonic.
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