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Post by exoticplantseller on Jun 28, 2016 8:26:22 GMT -5
Hi I wanted to try TC, it looks more rewarding. Is it easy to do? What do i need? And how do i do it? I have perfect little TC jars. I think that's what they are actuqlly made for. If you can help that would be amazing. Thanks
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Post by shoggoths on Jun 28, 2016 10:50:27 GMT -5
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Post by exoticplantseller on Jun 28, 2016 13:03:29 GMT -5
Thank you. I will look at them.
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Post by sokkos on Jun 29, 2016 8:27:31 GMT -5
I suggest you try starting off with seed first. Then try doing the classic African violet or tobacco experiments using leaf tissue before trying to use carnivorous plant tissue.
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Post by exoticplantseller on Jun 29, 2016 10:53:20 GMT -5
I suggest you try starting off with seed first. Then try doing the classic African violet or tobacco experiments using leaf tissue before trying to use carnivorous plant tissue. Ok thanks. I can't find information on this, and I know you have done it. my parents know nothing about where to get this stuff. I have perfect jars, but I don't know how to mix the gel or anything like that. Can you do like a series of pictures or make a video? That would really help me. thanks
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Post by sokkos on Jun 29, 2016 14:41:22 GMT -5
Unfortunately I don't have time at the moment to take a picture of every step. I know there are definitely videos on Youtube that show the process. Essentially you follow a recipe; first you dissolve everything you need in water, then adjust the pH, add agar according to the recipe, then put it in an autoclave (or pressure cooker) to sterilize. The recipes are all online. In my other tissue culture thread I've listed all the recipes as well as the sterilizing process for sundew seeds.
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Post by lloyd on Jun 29, 2016 16:07:24 GMT -5
There's also a TC section here. TC is very advanced and difficult. There are lots lots of far easier plants which will be more rewarding for you for at least a few years.
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Post by exoticplantseller on Jun 29, 2016 16:09:13 GMT -5
Unfortunately I don't have time at the moment to take a picture of every step. I know there are definitely videos on Youtube that show the process. Essentially you follow a recipe; first you dissolve everything you need in water, then adjust the pH, add agar according to the recipe, then put it in an autoclave (or pressure cooker) to sterilize. The recipes are all online. In my other tissue culture thread I've listed all the recipes as well as the sterilizing process for sundew seeds. Ok thanks for your help. I will keep looking for recipes and keep trying to understand it more.
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Post by exoticplantseller on Jun 29, 2016 16:10:16 GMT -5
There's also a TC section here. TC is very advanced and difficult. There are lots lots of far easier plants which will be more rewarding for you for at least a few years. Ok thanks, I want to try this because it is a challenge for me.
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Post by WillyCKH on Jun 29, 2016 19:31:42 GMT -5
There's also a TC section here. TC is very advanced and difficult. There are lots lots of far easier plants which will be more rewarding for you for at least a few years. Ok thanks, I want to try this because it is a challenge for me. I admire your braveness, TC is one of the advanced things that I'd fear getting my hands on...
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Post by exoticplantseller on Jun 29, 2016 19:47:56 GMT -5
Ok thanks, I want to try this because it is a challenge for me. I admire your braveness, TC is one of the advanced things that I'd fear getting my hands on... Why would you fear it? It is a challenge and you need a challenge once and a while. Some times you gotta step out of the box. Lol it does seem kind of confusing though lol
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Post by Tarantulalover on Jun 29, 2016 19:53:15 GMT -5
I don't have the time or space or really anything for tissue culture lol. It seems like it would take years to perfect doing it.
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Post by WillyCKH on Jun 29, 2016 20:09:23 GMT -5
I admire your braveness, TC is one of the advanced things that I'd fear getting my hands on... Why would you fear it? It is a challenge and you need a challenge once and a while. Some times you gotta step out of the box. Lol it does seem kind of confusing though lol keeping my plants happy is already challenging enough lol
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Post by sokkos on Jun 29, 2016 20:37:31 GMT -5
I was very fortunate to have an excellent mentor teach me TC (she spent her career working in agriculture and TC) and worked part time through school in a lab that routinely needed TC. You can learn the basics from any plant biology lab, so if you're in school at the moment, I'd strongly recommend you seek out such a lab to work or volunteer in. Even microbiology would be a good alternative just to learn how to make medium and keep things sterile. The principles you learn can apply to any plant you grow in TC. TC is very doable at home, however I think learning TC at school where you can use all the equipment is probably the easiest way to start. [Shameless plug: the University of Guelph has a very good 3rd year undergrad course on plant TC, and you actually get to do a lot of hands-on TC].
Just remember, almost any plant can be grown in TC, but not everything needs to be grown in TC.
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Post by WillyCKH on Jun 29, 2016 20:39:01 GMT -5
I don't have the time or space or really anything for tissue culture lol. It seems like it would take years to perfect doing it. I feel you... I know there's home-made recipes, simplified methods, and detailed instructions on the 'net, but like Lloyd said, there are many easier plants that will keep us happy for at least the next few years
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