I have been back into tissue culture for about 1 year now. Over that time, I have been slowly adding to the plants I have in culture. Most of the plants have been put into sterile culture from seeds but a few from flower stalks and leaf pulls. I purchased some seeds online but most of the material has been obtained from people through this board.
The cultures all appear to be stable right now and it looks like I will have more time in the new year to begin multiplying the plants. I will also get around to putting more plants in culture from my collection plus some of the extra seeds I have collected. I am also waiting on other seeds to germinate but it may be a lost cause if they were not fresh when I put them into culture.
I also lost most of the flytraps cultures from flowerstalks when my greenhouse went above 40 degrees in the summer. I am planning to monitor and control the greenhouse with an Arduino this growing season.
Nepenthes maxima N Lowii N veitchii N stenophylla
Heliamphora minor H nutans
Sarracenia "red tube" alata S flava x oreophila
Drosera binat D indica D peltata D auriculata D capensis "giant" D hilaris D binata "marston giant" D filiformis "California sunset" D. lowriei
Flytraps B52 DC XL Dentate Patches Red Dragon Typical Phalanx Flaming Lips Red Piranha Big Mouth
I tried TC with rare african violets...mostly went well until I had to transfer to growing out containers...guess I was not sanitary enough, turned brown and collapsed forever waiting to try TC again, with several plant species!
Post by snapperhead51 on Dec 17, 2015 18:03:37 GMT -5
Acclimatization of T/c plants is critical and often extremely hard to get right , each plant can need different methods to get acclimatized , soft plants are the worst indeed to get right , most orchids are very easy , most CP's are reasonably difficult , think the hardest is helis my self , and VFT's probably the easiest.
Post by amanitovirosa on Dec 17, 2015 19:52:00 GMT -5
...I got some TC Cephs off eBay a while back (know absolutely nothing about TC myself). Two batches, one completely died off and I got nothing but I was lucky with the other one and got a bunch of strikes. In total there were probably several hundred tiny TC Cephs, out of those I probably have about 40-50 that survived the transition to media and are growing. I'm really hoping for some mutations. Nice list, if you will be selling any I'd be interested in some. Cheers!
I have successfully deflasked VFT, Nepenthes and Heliamphora in the past. I have put all my Nepenthes inside under lights and only heat my greenhouse to keep it above freezing. When I get my greenhouse controls going in the spring, I will think about trying to grow some of the plants out. I have put some plants like the DC XL into multiplication medium and should have quite a few extras. These where seeds from a DC XL x DC XL so there is no telling whether these will be as large as a plant vegetatively propagated. I don't know about selling any of these plants but I am always up for trades. I do owe some people who sent me plant materials some of the offspring.
dvg: Sarra's safe inside
Mar 31, 2019 20:40:58 GMT -5
bonfield: I've decided to finally reveal my best-kept secret for growing healthy Neps: Just spit on them every few days, the enzymes in saliva help them to better absorb the fertilizer I spray them with!
Apr 1, 2019 14:24:31 GMT -5
dvg: Salivating up your drools?
Apr 1, 2019 16:30:07 GMT -5
dvg: Duped again on April Fool's!
Apr 1, 2019 16:30:45 GMT -5