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Post by H2O on Oct 20, 2015 14:26:29 GMT -5
Hey guys, I've been super busy lately but wanted to post this to get some opinions from the experts. Its a high quality GA3 growth regulator normally used for greenhouse crops but we were hoping to use it on some seeds (mostly Sarracenia). Anyone have any thoughts on how to mix this up and how to get roughly 250ppm and if it will work for seed application. If it does work anyone on this forum is welcome to as much as they want.
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Post by hal on Oct 20, 2015 16:44:40 GMT -5
I'll give it a shot and maybe Lloyd can check my math.
1,000,000/250 = 4000. So you have to mix it 1 to 4000 by weight
So for 1 gram of Ga3 you need 4 litres (4000 grams) of water. But it's only 40% GA3, so you need 4/0.4= 10 litres per gram. With 50g in the bottle you can mix 500 litres of the stuff. Should be enough for Gabe's VFTs or Maiden's Helis, although I doubt either needs any GA3.
Fun fact: In grade 7 I did a science fair experiment with Gibberelic Acid.
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Post by lloyd on Oct 20, 2015 17:12:35 GMT -5
Looks good, I'll check the math later. GA3 needs to be refrigerated.
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Post by shoggoths on Oct 20, 2015 18:36:38 GMT -5
I'll do it another way, you need to put 625 mg of ProGibb per litre of water to get a 250 ppm solution.
There's 400 000 mg of GA3 per 1 000 000 mg of ProGibb. You wish to get 250 mg of GA3 per litre of water. So 250 * 400 000 / 1 000 000 = 625 mg.
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Post by shoggoths on Oct 20, 2015 19:03:38 GMT -5
But, GA3 is not really soluble in water. The best is to disolve it in another solvent ... usually isopropyl alcohol then put the alcohol/GA3 mix in water. The problem, isopropyl alcohol can be toxic to seeds so you need to use the minimum amount of alcohol. Also, you will get better dissolution if you heat the alcohol. To do so, put the container with the alcohol in hot water NOT over flame.
Dissolving 625 mg of ProGibb will not be easy, I would do a smaller volume like 62.5 mg / 100 ml.
Good luck.
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Post by lloyd on Oct 20, 2015 21:00:25 GMT -5
Everybody above is exactly correct. Here's the way I do it.
GA3 is even more unstable in solution than in powder so I usually make only 40 cc of 250 PPM GA3 at a time, which requires 10 mg. of pure GA3. Since this stuff is only 0.4 GA3 by weight, you have to multiply by 2.5. So for 40 cc of 250 PPM GA3, you would need 25 mg. I have a scale that is accurate to 0.1 mg. so I would just weigh out 25 mg. which is a "pinch". This is really easy to dissolve in a drop or two of 99% Isopropyl alcohol, or if you live in Quebec, 90% Ethanol (which may be less toxic). Then you add distilled water to make up 40 cc which will last a long time. Keep all GA3 in the fridge.
Questions: has this stuff been stored at appropriate temperatures? How old is it? Is there a "Best-before" date?
Thanks for the offer, Justin.
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Post by snapperhead51 on Oct 23, 2015 5:10:39 GMT -5
yes I use .5 of a GM in 250 ML water and use a auto stir for approx 4 to 5 hrs to mix the granulate power to make it full mixed , keep all materials in fridge out of light , as it will De-grade quickly
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