|
Post by sokkos on Apr 24, 2014 9:28:17 GMT -5
I wanted to share some pictures of the sundew cultures that I have. I used MS media because it was readily available. The half strength dilution was based on what others have done in the literature. I have also used 1/3 strength with good results. Seed sterilization followed protocols from literature and also from other online sources. Media: half strength MS basal salts, full strength Gamborg B5 vitamins, 3% v/w sucrose, 0.6-0.8% v/w agar, pH 5.5-5.7 Seed sterilization: 20% commercial bleach + Tween20 (6min), 3x 5min rinse in water D. capensis Initial plant received from 31drew31 in 2011 Seeds collected: 2014-Jan-30 Plated: 2014-Feb-22 First sprouts: 2014-Mar-3 Sub-cultured: 2014-Apr-21 D. tokaiensis Seeds received from Shoggoths 2014-Jan Plated 2014-Jan-30 Last subculture: 2014-Mar-22 Beginning to root: 2014-April D. binata Seeds received from Shoggoths 2014-Jan D. spatulata Seeds received from Shoggoths 2014-Jan The next two came from another lab. The technician gave them to me after he heard I was interested in sundews. D. rotundifolia Initial cultures received: 2013-September Last subculture: 2014-Apr-21 D. communis (I believe this is mislabeled and should actually be D. spatulata) Initial cultures received: 2013-September Last subculture: 2014-Apr-21
|
|
|
Post by lloyd on Apr 24, 2014 15:28:20 GMT -5
Very nice. Are the petri dishes sealed? Are they just regular plastic petri dishes?
|
|
|
Post by sokkos on Apr 24, 2014 16:21:12 GMT -5
Yes they are sealed with parafilm. They are regular plastic petri dishes 100x25mm.
|
|
|
Post by canuk1w1 on Apr 24, 2014 18:30:13 GMT -5
Great job! I'm hoping to graduate to trying tissue culture in a year or two...
|
|
|
Post by Devon on Apr 24, 2014 19:39:28 GMT -5
Great results!
|
|
|
Post by dvg on Apr 25, 2014 14:12:10 GMT -5
Very impressive! Best of luck with them all! dvg
|
|
|
Post by shoggoths on Apr 26, 2014 8:29:01 GMT -5
Good job Sokkos. Thank you for the picture. They really give me a kick to start some new one.
|
|
|
Post by Apoplast on May 4, 2014 10:22:05 GMT -5
Wow! And so nicely ordered in rows and columns too. That's pretty great!
|
|
|
Post by LAKJP on May 4, 2014 10:24:17 GMT -5
They look good
|
|
|
Post by Dennis A(cook1973) on May 18, 2014 12:13:30 GMT -5
Yes they are sealed with parafilm. They are regular plastic petri dishes 100x25mm. I got some drosera dews via TC are the roots very noticable? Mine are in LFS right now and I cannot see the root so I got them in thier own baggie with some RO water and sealed under light to give then high humidity I need to wait til the end of the month to get paid and get something to pot them in and more baggies will they make it until then, I ran out of planting containers!! will they be ready to pot then by that I mean will the roots be long enough to pot the plants by then and go back in the baggie for more humidity?? I was sent VFT seedlings too via TC, but had to pot them right away and put them in a baggie with RO water inside a container(a cleaned 1 pound butter tub)they look like tiny little plants,even forming tiny traps! Any advice will be appreciated
|
|
|
Post by sokkos on Jul 27, 2014 9:10:20 GMT -5
UPDATE: July 27th. Just a few new pictures. If anyone wants any of these plants, let me know and I can propagate some for you. Venus fly trap, seeds from Dvg. Sprouted in less than a month. Drosera auriculata, seeds from Shoggoths Drosera sessifolia, seeds from Shoggoths
|
|
|
Post by hal on Jul 27, 2014 9:55:20 GMT -5
Looking good. The tuberous dew seeds that' shoggoths sent me a few months ago have just sprouted using traditional methods.
|
|
|
Post by ng on Jul 27, 2014 11:27:11 GMT -5
Awesome work sok! Ide love to try some of those odd ball sundews when they mature!
|
|
|
Post by Dennis A(cook1973) on Jul 27, 2014 12:21:34 GMT -5
Looks like you got TC nailed
|
|
|
Post by sokkos on Sept 16, 2014 14:06:44 GMT -5
UPDATE Sept 16, 2014 I've been busy with school so I haven't had as much time as earlier before to work on my cultures. Some newly established species: D. aliciae, seed from Maiden D. "Pretty Rosette," seed from Maiden D. aliciae, seed from BCP just starting to germinate D. capensis alba, seed from ng D. capensis Giant form, seed from BCP. I'm SUPER excited about this species! Can't wait to transfer them to pots and see how big they can get! Not a newly established culture per se, but VTF seed from dvg
|
|