I wanted to share some pictures of the sundew cultures that I have. I used MS media because it was readily available. The half strength dilution was based on what others have done in the literature. I have also used 1/3 strength with good results. Seed sterilization followed protocols from literature and also from other online sources.
Media: half strength MS basal salts, full strength Gamborg B5 vitamins, 3% v/w sucrose, 0.6-0.8% v/w agar, pH 5.5-5.7 Seed sterilization: 20% commercial bleach + Tween20 (6min), 3x 5min rinse in water
D. capensis Initial plant received from 31drew31 in 2011 Seeds collected: 2014-Jan-30 Plated: 2014-Feb-22 First sprouts: 2014-Mar-3
D. tokaiensis Seeds received from Shoggoths 2014-Jan Plated 2014-Jan-30
Last subculture: 2014-Mar-22
Beginning to root: 2014-April
D. binata Seeds received from Shoggoths 2014-Jan
D. spatulata Seeds received from Shoggoths 2014-Jan
The next two came from another lab. The technician gave them to me after he heard I was interested in sundews.
D. rotundifolia Initial cultures received: 2013-September Last subculture: 2014-Apr-21
D. communis (I believe this is mislabeled and should actually be D. spatulata) Initial cultures received: 2013-September Last subculture: 2014-Apr-21
Yes they are sealed with parafilm. They are regular plastic petri dishes 100x25mm.
I got some drosera dews via TC are the roots very noticable? Mine are in LFS right now and I cannot see the root so I got them in thier own baggie with some RO water and sealed under light to give then high humidity I need to wait til the end of the month to get paid and get something to pot them in and more baggies will they make it until then, I ran out of planting containers!! will they be ready to pot then by that I mean will the roots be long enough to pot the plants by then and go back in the baggie for more humidity?? I was sent VFT seedlings too via TC, but had to pot them right away and put them in a baggie with RO water inside a container(a cleaned 1 pound butter tub)they look like tiny little plants,even forming tiny traps! Any advice will be appreciated
dvg: Sarra's safe inside
Mar 31, 2019 20:40:58 GMT -5
bonfield: I've decided to finally reveal my best-kept secret for growing healthy Neps: Just spit on them every few days, the enzymes in saliva help them to better absorb the fertilizer I spray them with!
Apr 1, 2019 14:24:31 GMT -5
dvg: Salivating up your drools?
Apr 1, 2019 16:30:07 GMT -5
dvg: Duped again on April Fool's!
Apr 1, 2019 16:30:45 GMT -5