My D. derbyensis in TC is coming along. The first one is already dime sized and there are 4 others at various levels in the agar. Seems to be fairly rapid growth. I have no experience with the petiolaris group except rapidly killing the petiollaris that Tom sent me. I hope I can get these guys going as it seems nobody else has them in their grow-lists.
I have deflasked two derbyensis and they are looking fairly good. I was thinking of taking one of the ones still in TC and transferring some leaf pullings to a multiplication medium to try and propagate them. Anybody have much experience with the petiolaris group?
Also I was thinking of starting some more TC from seed over the winter. Maybe some of the weirder sundews. Any suggestions? I will probably order from Triffid Nurseries in UK or maybe rareroticseeds.
One derbyensis went to Tom (who sent me a nice P. colimensis & D. paradoxa). One is doing nicely deflasked with lots of white hair.
Last night I had a "Vraev Moment". I had left two derbyensis in TC until they sort of went crazy splitting the agar with a root ball. I ruthlessly pulled about 10 or so leaves off and put them in a multilication medium. Hopefully they will grow. Then I deflasked the two plants which turned a dark green and seemed to lose some turgor. I hope they won't die. I'm afraid to look at them. I'll wait a few days until they hopefully are doing Ok. I may have left them in TC too long or maybe the leaf pulling was too traumatic.
came across these papers at British Library Direct, PM'd ya the link
thought that maybe there might be something useful to ya buried in them somewhere
I dont do TC, but thought the first and last sounded especially interesting
1 Rapid in vitro multiplication of Drosera indica L.: a vulnerable, medicinally important insectivorous plant Jayaram, K. Prasad, M. N. PLANT BIOTECHNOLOGY REPORTS 2007 VOL 1; NUMBER 2, page(s) 79-84 SPRINGER SCIENCE + BUSINESS MEDIA Abstract: An efficient protocol is described for rapid in vitro multiplication of the vulnerable medicinal herb Drosera indica L. by enhanced axillary bud proliferation from shoot tips as explants. In order to standardize in vitro multiplication of D. indica, the effects of different strengths of Murashige and Skoog (MS) medium (1/4, 1/3, 1/2 and full strength), different percentages of sucrose (1, 2 and 3%), various pH (3.7, 4.7, 5.7 and 6.7) and MS basal medium fortified with different concentrations of zeatin (Z), kinetin (KN) (0.1, 0.5, 1.0 and 2.0Â mg/l) and 6-benzylaminopurine (BA) (0.01, 0.05 and 0.1Â mg/l) were tried. Multiple shoot production was independent of different strengths of MS, various percentages of sucrose and also when pH was altered. Although the number of multiple shoots deve... 2 Variations of naphthoquinone levels in micropropagated Drosera species in vitro, under greenhouse and outdoor growth conditions Wawrosch, C. Vackar, E. Grauwald, B. Krenn, L. SCIENTIA PHARMACEUTICA 2005 BAND 73; HEFT 4, page(s) 251-262 OSTERREICHISCHE APOTHEKER VERLAGSGESELLSCHAFT Abstract: 3 Rapidly in vitro multiplied Drosera as reliable source for plumbagin bioprospection Jayaram, K. Prasad, M. N. V. CURRENT SCIENCE -BANGALORE- 2005 VOL 89; NUMB 3, page(s) 447-448 CURRENT SCIENCE ASSOC / INDIAN ACADEMY OF SCIENCES Abstract: 4 Secondary Metabolites in In Vitro Cultured Plants of the Genus Drosera Marczak, L. Kawiak, A. Lojkowska, E. Stobiecki, M. PHYTOCHEMICAL ANALYSIS 2005 VOL 16; NUMB 3, page(s) 143-149 JOHN WILEY & SONS LTD Abstract: 5 Application of RAPD in the Determination of Genetic Fidelity in Micropropagated Drosera Plantlets Kawiak, A. Lojkowska, E. IN VITRO CELLULAR AND DEVELOPMENTAL BIOLOGY PLANT 2004 VOL 40; NUMB 6, page(s) 592-595 CABI PUBLISHING Abstract: 6 Application of RAPD in the Determination of Genetic Fidelity in Micropropagated Drosera Plantlets Kawiak, A. Lojkowska, E. IN VITRO CELLULAR AND DEVELOPMENTAL BIOLOGY PLANT 2004 VOL 40; PART 6, page(s) 592-595 CABI PUBLISHING Abstract: 7 Extracellular Matrix in Early Stages of Direct Somatic Embryogenesis in Leaves of Drosera spathulata Bobak, M. Samaj, J. Hlinkova, E. Hlavacka, A. Ovecka, M. BIOLOGIA PLANTARUM 2003 VOL 47; NUMBER 2, page(s) 161-166 Kluwer Academic Publishers Abstract: Leaves from mature in vitro grown plants of Drosera spathulata Labill. regenerated new plantlets on solid induction medium in light. Especially vascular sheath parenchyma cells located close to basal part of tentacule showed high embryogenic potential. Proembryoids arrising from the tentacule base part were visible by scanning electron microscopy. Their surface cells were linked and covered with thin external, fibrilar network representing an extracellular matrix (ECM). Proembryogenic surface cells were later connected by coarse strands of fibrils. Young protoderm was formed arround globular embryoids and its cells were characterized by "brain-like" surface structure. However, the surface of fully developed protodermal cells was practicaly smooth and cells were stick to each other very tig... 8 Micropropagation of Drosera peltata, a Tuberous Sundew, by Shoot Tip Culture Kim, K. S. Jang, G. W. PLANT CELL TISSUE AND ORGAN CULTURE 2004 VOL 77; NUMBER 2, page(s) 211-214 Kluwer Academic Publishers
Thanks, Butch. I'm going to look at those articles on line as you suggest. I wish there were a local course on such material. I can't help thinking it would be so much easier to have a mentor who could actually show you what to do in a proper lab. In the meantime, I'll keep puttering along.
Guru... perhaps a too strong word for me! I have a good basis and worked a little in the field, but im far from being an expert... I'm sure there's others as good if not better than me, such as Rick Keehn, Lloyd, Sdeering, Purpman... TC has been placed a bit on the side for a few moment, until i find the time and money to reinstall a modest mini-lab for fun...
------------------------------------------------- "C'est une triste chose de songer que la nature parle et que le genre humain n'écoute pas." -Victor Hugo
I somehow seemed to have killed off my D. derbyensis that I recently deflasked. I took some leaf pullings for a multiplication medium and maybe that traumatized the plants. Luckily two survived one for me and one for Tom. I'll see how the leaf pullings do, so far they don't look like much. I have some VFT seeds in TC (from Jay), so far no germination yet and some problems with contamination, I think endogenous.
dvg: Sarra's safe inside
Mar 31, 2019 20:40:58 GMT -5
bonfield: I've decided to finally reveal my best-kept secret for growing healthy Neps: Just spit on them every few days, the enzymes in saliva help them to better absorb the fertilizer I spray them with!
Apr 1, 2019 14:24:31 GMT -5
dvg: Salivating up your drools?
Apr 1, 2019 16:30:07 GMT -5
dvg: Duped again on April Fool's!
Apr 1, 2019 16:30:45 GMT -5