I just got an order from the chemical supply firm I mentioned in Quebec. It was for potassium citrate, about a third the price of Phytotech. Supposedly when you mix it with citric acid it reduces phenolic bleeding. Of course someone at Canada Post had to rip it open and check for drugs. Luckily only a bit was lost.
Just put all my Rareexoticseeds into tissue culture. I'm dubious that I'll get anything but anyway it's pretty low maintenance sitting under the lights. Some of the seeds were so small I could hardly pick them up with my best forceps.
For tiny seeds, as sundews and Pings, I get better result by trying with my scalpel tip as a spathula, or flame your scalpel util red-hot and cool it on the surface media. you should 'harvest' by the same time some melted agar which will help you to 'gather' some seeds, and place the whole thing onto your media.
You could also 'suspend' them into a water solution, but you would need a pipette or a very nice syringe to do this.
------------------------------------------------- "C'est une triste chose de songer que la nature parle et que le genre humain n'écoute pas." -Victor Hugo
Lots of supplies and equipment but not amazing results so far. S. purpurea are going crazy from seed. D. madagascarensis small but cute on the agar. The infamous "mixed sarr seeds" with mixed results, initial ? internal contamination with mould (now cleared) and some stunting. Darlingtonia seeds growing not too bad but slow. S. oreophila germinating well. Lots of other seeds sitting there, sadly, doing nothing.
My latest is my own mix with 1/2 MS with vit's., IAA, BAP, potassium citrate/citric acid, activated charcoal, agar, sucrose, pH 5.8, 0.1 PPM, distilled water. I put a ceph leaf in a few weeks ago and it has to sit in the dark. So far no growth but no contamination or phenolic bleeding either.
I noticed in my S. purp and darlingtonia jars there is what looks like a monoculture of a green algae on the side of the glass. It doesn't show signs of taking everything over and doesn't seem pathogenic. It came after I transferred the seedlings which was done at different days. Could it be a growth of free plant cells released by tissue damage on handling? Or some internal commensural organism released in the same way? It hasn't shown up in the other 2 dozen or so jars so it seems related to these two species.
I had mould before. In my limited experience, mould grows on the surface of the agar and has a powdery, wrinkled pastel-green appearance and takes over everything. This is a bright chlorophyll green and it's between the agar and the glass and shows no sign of aggressive spread. Mould shouldn't have chlorophyll.
I was just looking in the TC bottles I gave up on and saw a very small Drosera derbyensis growing. It was from a batch of seeds from Triffid in England. I must have put it in TC 3 months ago. I'll post again as things develop.
dvg: Sarra's safe inside
Mar 31, 2019 20:40:58 GMT -5
bonfield: I've decided to finally reveal my best-kept secret for growing healthy Neps: Just spit on them every few days, the enzymes in saliva help them to better absorb the fertilizer I spray them with!
Apr 1, 2019 14:24:31 GMT -5
dvg: Salivating up your drools?
Apr 1, 2019 16:30:07 GMT -5
dvg: Duped again on April Fool's!
Apr 1, 2019 16:30:45 GMT -5