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Post by Flytrap on Sept 1, 2006 10:26:39 GMT -5
www.omnisterra.com/botany/cp/slides/tc/tc.htmTissue culture in the Home Kitchen it's not as hard as you might think!
A slide show by Rick Walker
Why bother with Tissue Culture (TC)?
faster propagation (perhaps 10x) - reduces demand for wild collected plants. no worry about fungus, pests, etc. long term maintainance of plants (stick 'em in the fridge!) IDENTICAL clones of horticultural varieties easily generated (actually, the last point is not quite true. Scott Hyndman, marva@nebula.ispace.com, informs me that Clonal integrity is influenced by stress factors, genotype, culture age, cutting and transfering technique, and numerous other factors still being investigated in the voluminous scientific literature on just this fascinating aspect of plant tissue culture alone.)
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Post by japa2222 on Nov 21, 2006 21:04:28 GMT -5
Just a word of warning. I wouldn't recommend 15 psi because the sucrose will most likely carmelize. Also it's much easier to find a 10 psi pressure cooker at wal-mart. It should be fine but you can add about 10 minutes onto cooking time in order to be sure. You do the same thing for mushroom cultures.
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Post by sdeering on Nov 21, 2006 21:30:03 GMT -5
It all depends on what elevation you are at I use 15 psi. The higher the elevation the more pressure ( weight you need). I think the number is 121*C to kill off everything. There is also a term used by the mushroom growers as pasteurizing. This is done at a lower temp. The beneficial bugs remain alive. This is used usually when using a natural media like straw. Stephen
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Post by japa2222 on Nov 21, 2006 22:17:39 GMT -5
You are right about the elevation factor on water's boiling point. However either way you choose to do it, it all depends on time at a certain temperate because you are basically doing the same thing, removing contaminants from the agar. Also with mushroom cultivation there are no beneficial bugs, only nutrients one of them being a sugar which will carmelize if your not careful about the psi. Here are various psi's in relation to temp. PSI Temp(in F) 1 212 3 220 5 228 10 240 15 250 20 259 25 267
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Post by vraev on Nov 22, 2006 1:32:33 GMT -5
reallly interesting read. very informative but at the same time brought back a lot of memories for me. I did all those techniques in my first year bilogy labs for plating bacterial colonies. Similar techniques in sterilizing the loop and holding the stuff in the hand and so on. Its not sooo hard as some might think. Pretty easy and FUN.
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